Journal: Antioxidants

Article Title: Therapeutic Potential of Cranberry Proanthocyanidins in Addressing the Pathophysiology of Metabolic Syndrome: A Scrutiny of Select Mechanisms of Action

doi: 10.3390/antiox14030268

Figure Lengend Snippet: Effect of PAC on insulin resistance, fasting lipid profile, lipoprotein composition, and inflammatory circulating biomarkers. At weeks 0, 6, and 10, ( A ) fasting glycemia and ( B ) fasting insulinemia was measured, enabling the calculation of the ( C ) Homeostatic model assessment of insulin resistance (HOMA-IR). At weeks 0, 6, 10, and 12, ( D ) fasting triglyceridemia and ( E ) fasting total cholesterolemia were measured. Results are presented as means ± SEM for n = 6–12 mice/group. At week 12, the lipid profile was further investigated to determine levels of ( F ) HDL-cholesterol and ( G ) non-HDL-cholesterol. Results are presented as means ± SEM for n = 4 pooled plasma/group. At week 12, plasma from n = 8 mice/group was collected and pooled for lipoprotein determination. Isolated fractions were first obtained via fast-protein liquid chromatography (FLPC) and then were analyzed to reveal content in ( H ) total cholesterol and ( I ) esterified cholesterol, as described in Materials and Methods. Circulating ( J ) adiponectin was measured at week 10. Circulating ( K ) interleukin-1-alpha (IL1A), ( L ) Monocyte Chemoattractant Protein-1 (MCP1), and ( M ) Tumor Necrosis Factor-alpha (TNFA) were obtained at week 12. Results are presented as means ± SEM for n = 6–8 mice/group. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. Chow; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. HFHF mice.

Article Snippet: Plasma samples ( n = 8 mice/group) obtained at week 10 were used to measure high molecular weight (HMW) adiponectin concentrations with the Mouse HMW and Total Adiponectin ELISA kit (ALPCO, Salem, NH, USA).

Techniques: Clinical Proteomics, Isolation, Fast Protein Liquid Chromatography

Journal: ACS Omega

Article Title: Oral Administration of the Adiponectin Receptor 1 Agonistic Dipeptide Tyr-Pro Prevents Hyperglycemia in Spontaneously Diabetic Torii Rats

doi: 10.1021/acsomega.4c09030

Figure Lengend Snippet: Involvement of Tyr-Pro in glucose uptake-related signaling pathways, AdipoR1-mediated Signaling Pathway, and insulin signaling pathway in SDT Rats. Plasma insulin (A) and adiponectin (B) levels in SDT rats euthanized at 29 weeks of age were measured using ELISA insulin/adiponectin assay kits. The protein expression of (C) AdipoR1, (D) p -AMPK/AMPK, (E) GLUT4, and (F) p -IRS-1(Tyr608)/IRS-1 and p -IRS-1(Ser612)/IRS-1 in skeletal muscle tissue of SDT rats euthanized at 29 weeks of age was measured using Wes analysis. Protein expression was normalized by the electropherogram peak area of total protein in each lane. The chemiluminescent signal was displayed as a virtual blot-like image, and an electropherogram was generated based on the molecular weight. Results are presented as the mean ± SEM ( n = 3–4). Statistical analyses between the two groups were evaluated by Student’s t -test at p < 0.05, p < 0.01, p < 0.001, and N . S .: not significant at p < 0.05.

Article Snippet: Plasma adiponectin levels were determined using a rat adiponectin ELISA kit (HMW adiponectin, Lot: 634–13071, FUJIFILM Wako Shibayagi Co.) according to the manufacturer’s instructions.

Techniques: Protein-Protein interactions, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Expressing, Generated, Molecular Weight

Journal: Journal of Biological Engineering

Article Title: Sustainable production of multimeric and functional recombinant human adiponectin using genome-edited chickens

doi: 10.1186/s13036-024-00427-2

Figure Lengend Snippet: Sustainability of multimeric patterns in recombinant human adiponectin (hADPN) across subsequent generations of ADPN knock-in (KI) genome-edited chickens ( A ) Pedigree of ovalbumin ( OVA ) ADPN KI genome-edited chickens across generations. ( B ) Quantitative analysis of total ADPN protein abundance in genome-edited hens across generations. ( C ) Quantitative analysis of high molecular weight (HMW) ADPN protein abundance of genome-edited hens across generations. Statistical analysis was performed using one-way ANOVA ( n = 3). Significant differences are indicated as “ns”, no significance. ( D , E ) Western blotting analysis of ADPN in OVA ADPN KI chicken egg white under non-reducing and reducing conditions. Arrows indicate the forms of ADPN, including HMW, hexamer (medium), trimer (low), and monomer

Article Snippet: The quantities of total ADPN and HMW ADPN from OVA ADPN KI EW were measured using human total ADPN quantikine ELISA kit (DRP300; R&D systems, Minneapolis, MN, USA) and human HMW ADPN quantikine ELISA kit (DHWAD0; R&D systems) respectively.

Techniques: Recombinant, Knock-In, Quantitative Proteomics, High Molecular Weight, Western Blot

Journal: Journal of Biological Engineering

Article Title: Sustainable production of multimeric and functional recombinant human adiponectin using genome-edited chickens

doi: 10.1186/s13036-024-00427-2

Figure Lengend Snippet: Comparative analysis of the multimeric composition of recombinant hADPN ( A ) Detection of multimeric recombinant hADPN derived from OVA ADPN KI chicken egg white (EW)-derived hADPN and other commercial recombinant hADPNs derived from human embryonic kidney (HEK) 293 cells with a C-terminal FLAG tag, High-Five (Hi-5) cells, and human serum (positive control) by western blotting analysis under non-reducing condition. ( B ) Detection of reduced EW-derived recombinant hADPN and other commercial recombinant hADPNs derived from HEK293 cells, Hi-5 cells, and human serum by western blotting analysis. Arrows indicate HMW, hexamer (medium), trimer (low), and monomer forms of hADPN.

Article Snippet: The quantities of total ADPN and HMW ADPN from OVA ADPN KI EW were measured using human total ADPN quantikine ELISA kit (DRP300; R&D systems, Minneapolis, MN, USA) and human HMW ADPN quantikine ELISA kit (DHWAD0; R&D systems) respectively.

Techniques: Recombinant, Derivative Assay, FLAG-tag, Positive Control, Western Blot

Journal: Journal of Biological Engineering

Article Title: Sustainable production of multimeric and functional recombinant human adiponectin using genome-edited chickens

doi: 10.1186/s13036-024-00427-2

Figure Lengend Snippet: Comparative analysis of endoplasmic reticulum (ER) chaperone gene transcription in the adipose tissue, oviduct magnum of wild-type (WT) and ADPN KI hens, and HEK293 cells ( A − D ) mRNA expression levels of Ero1-Lα , DsbA-L , ERP44 , and PDI in the adipose tissue and oviduct magnum of WT and ADPN KI hens compared with HEK293 cells. Samples from WT hens are denoted as WT adipose tissue and WT oviduct magnum, while samples from ADPN KI hens are labeled as ADPN adipose tissue and ADPN oviduct magnum. Statistical analysis was performed using one-way ANOVA ( n = 3). Different superscript letters indicate significant differences between groups ( p < 0.05)

Article Snippet: The quantities of total ADPN and HMW ADPN from OVA ADPN KI EW were measured using human total ADPN quantikine ELISA kit (DRP300; R&D systems, Minneapolis, MN, USA) and human HMW ADPN quantikine ELISA kit (DHWAD0; R&D systems) respectively.

Techniques: Expressing, Labeling